Margarita Karkaletsi

Dynamic individual with hands-on experience in Human Genetics and talent for navigating challenges. Brings strong problem-solving skills and proactive approach to new tasks. Known for adaptability, creativity, and results-oriented mindset. Committed to making meaningful contributions and advancing organizational goals.

Training in the following techniques:

Department of Genetics and Molecular Biology, Bioiatriki Healthcare group

· SNP array (Cytoscan 750K array Affymetrix platform) in Prenatal and Postnatal Diagnosis

Alfa Lab, Genetics and Molecular Biology Centre

· Array-CGH (8x60 Agilent Platform) in Prenatal and Postnatal Diagnosis

· Preimplantation Genetic Screening with array-CGH

(8x60 Agilent Platform)

· FISH in sperm, CVS, amniotic fluid, products of conception and peripheral blood samples

· Sperm apoptosis

Department of Genetics and Molecular Biology, Mitera Maternity, Gynecological, Pediatric and General Hospital, Athens

· Conventional cytogenetic techniques for prenatal and postnatal diagnosis including cultures of CVS, amniotic fluid, products of conception and peripheral blood

· G-Banding

· DNA extraction

· Electrophoresis

· PCR

1 . Sismani C, Donoghue J, Alexandrou A, Karkaletsi M , Christopoulou S, Konstantinidou AE, Livanos P, Patsalis PC, Velissariou V. A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus Gene. 2013 Aug 22. doi:pii:S0378-1119(13)01062-710.1016/j.gene.2013.08.032. [Epub ahead of print]

2 . Genetic causes of infertility. Μ.Κarkaletsi , A. Chatzaki, V.Velissariou. Medical Anthology Issue 15 July- September 2012

3 . Genetic causes of increased nuchal translucency. Α. Spring, G. Christopoulou., T. Tseva, S. Christopoulou., Z.Donoghue, Μ. Karkaletsi, Ε. Manisali, V. Velissariou Medical Anthology Issue 15 July- September 2012

4 . Prenatal Diagnosis of Chromosomal Abnormalities. Α. Spring, G. Christopoulou., T. Tseva, S. Christopoulou., Z.Donoghue, Μ. Karkaletsi, Ε. Manisali, V. Velissariou Medical Anthology Issue 15 July- September 2012

5 . V. Velissariou, S. Christopoulou, J.Donoghue , G. Christopoulou, Μ. Κarkaletsi , Ε. Μanisali, Α. Spring, T. Τseva, Α. Chatzaki, D. Apostolopoulou, D. Bouzarelou ,S. Maragkou, Α. Stavrou. Prenatal diagnosis of sex chromosome abnormalities in first trimester pregnancies. Obstretics and Gynecology Issues, 2009, volume ΚΓ΄, issue 3, 181-186

6 . Christopoulou S, Christopoulou G, Hatzaki A, Hatzipouliou A, Donoghue J, Karkaletsi M, Kaminopetros P, Sifakis S, Velissariou V. The replacement of cytogenetic analysis of chorionic villi sampling direct preparation samples by quantitative fluorescence PCR. Gynecologic and Obstetric Investigation 2009, accepted for publication 3 June, 2009.

7 . Sifakis S, Karkaletsi M , Christopoulou S, Donoghue J, Kaminopetros P, Konstantinidou T, Velissariou V. Distinctive pattern of first trimester maternal serum biochemical markers in a trisomy 22 pregnancies. Prenat Diagn. 2008 Dec;28(12): 1174-6.

8. M.Karkaletsi, S. Christopoulou, Z. Donoghue, G. Christopoulou, S. Sifakis, P. Kaminopetros, A. Chatzaki, V. Velissariou. 6 cases of trisomy 22 in prenatal diagnosis: first trimester biochemical markers and ultrasound findings. Obstretics and Gynecology Issues, 2008, volume ΚB΄, issue 1, 21-25.

9. Kitsiou-Tzeli S, Sismani C, Karkaletsi M, Florentin L, Anastassiou A, Koumbaris G, Enangelidou P, Agapitos E, Patsalis PC, Velissariou V.Prenatal diagnosis of a de novo partial trisomy 10p12.1-12.2 pter originating from an unbalanced translocation onto 15qter and confirmed with array CGH.Prenat Diagn. 2008 Aug;28(8):770-2. PMID: 18615545 [PubMed - indexed for MEDLINE]

· Conventional Cytogenetics and Cytogenomics, Methods and Protocols. M.Karkaletsi

Postgraduate courses in Diagnostic Laboratory Principles,

Faculty of Medicine, National and Kapodistrian University

of Athens, 2022-2023

· Genetic causes of infertility . Μ.Karkaletsi, V.Velissariou. MHTERA Hospital, January 2011

1. Non-familial paracentricinversion inv(5)(q15q23.2) in the greek population: “innocent” polymorphism or a finding with clinical significance? Karkaletsi M., Lagou M., Rompola M., Neroutsou R. Velissariou V., Pantazidis G.Conference of Panhellenic Association of Medical Geneticists, 2024

2 . Investigation of a de novo complex intrachromosomal X-chromosome rearrangement in a girl with primary amenorrhea. Sarantis Gagos, Carolina Sismani, Alexandrou Angelos, Ketoni Andria, Christina Ifanti, Eleni Dragona, Margarita Karkaletsi , Voula Velissariou. European Human Genetics Virtual Conference 2021.

3. Molecular karyotype as a diagnostic tool in pregnancies with ultrasound findings as well as in low risk pregnancies. Sachinidou F., Billy C.,Panou E., Bouzarelou D., Gali E., Karkaletsi M ., Manisali E., Donoghue Z., Florentin L. 2ο Conference of Panhellenic Association of Medical Geneticists, 2016

4. Nuchal translucency and aneuploides. Α. Spring, G. Christopoulou., S. Christopoulou., J.Donoghue, Μ. Karkaletsi, Ε. Manisali, V. Velissariou Α. 1ο Conference of Panhellenic Association of Medical Geneticists, 2013

7 . Prenatal diagnosis of a de novo derivative chromosome 15 with extra genetic material derived from chromosome 10p. M.Karkaletsi , A.Hatzipouliou, C.Bili, F.Sahinidou, S.Kitsiou, A.Anastasiou, V.Velissariou.6th European Cytogenetics Conference, July 2007, Istanbul

8 . Trisomy 22 and biochemical markers in first trimester. S.Christopoulou, M.Karkaletsi , A.Hatzipouliou, J.Donoghue, E.Manisali, S.Sifakis, V.Velissariou. 6th European Cytogenetics Conference, July 2007, Istanbul

9 . QF-PCR: reliable and accurate for the rapid detection of the most common fetal chromosomal abnormaliities in the first trimester. G.Christopoulou, A.Hatzaki, S.Christopoulou, A.Chatzipouliou, J.Donoghue, M.Karkaletsi , V.Velissariou. ESHG, June 2007, Nice

10. 4 cases of trisomy 22 in prenatal diagnosis. M.Karkaletsi , S. Christopoulou, A.Chatzipouliou, Z. Donoghue, E.Manisali, S.Sifakis, V.Velissariou. Bioscience in 21ο century, April 2006, Athens

11 . Maternal uniparental heterodisomy 16 in a fetus with confined placental trisomy 16 mosaicism. Manolakos E., Karkaletsi M , Karadimas Ch., Papathanakos P., Agapitos E., Velissariou V. 6th Balkan Meeting on Human Genetics, August 2004, Thessaloniki

12 . Prenatal diagnosis of chromosomal abnormalities in the first trimester. J.Donoghue, A.Hatzipouliou, S.Christopoulou, E.Manolakos, M . Karkaletsi , V.Velissariou, V.Dedoulis, P.Kaminopetros, A.Komis, I.Sideris, A.Stavrou, I.Vamvakaris.6th Balkan Meeting on Human Genetics. August 2004, Thessaloniki